Systemic Inflammation, Elevated hs-CRP, and Oral Bacterial Pathogens Decrease After Two Years of Natural Dental Health Treatments: A Case Study

Heart Disease Begins in the Mouth

High sensitivity C-reactive protein (hs-CRP) is the most clear, quantifiable, and readily accessible marker of the oral-systemic connection. We present a case study of an otherwise healthy 62-year-old woman with elevated hs-CRP who had abundant numbers of pathogenic oral bacteria. A natural dental health treatment over two years lowered hs-CRP and lowered oral pathogens. Given the links between oral disease, inflammation, and heart disease, it is wise to test for oral dysbiosis in patients presenting with an elevated hs-CRP.

Case Study

A 62-year-old woman, named “Linda” for the purposes of this case, presented to her family physician for her annual check-up. The integrative medical doctor did the usual battery of tests on Linda, including blood sugar, cholesterol, and hs-CRP. Linda said she was feeling fine and didn’t report any major issues with her health, nor signs of gum disease. Linda’s lab tests came back and she had high levels of serum hs-CRP, at 6.3 mg/L (reference range <1.0 ).

hs-CRP is an acute phase reactant produced by the liver. It elevates due to infection, inflammation, or trauma. hs-CRP has been established as an independent predictor for coronary artery disease1 and a key biomarker of systemic inflammation.2 hs-CRP correlates with periodontal disease severity, and decreases after nonsurgical periodontal therapy.3  Finally, an association between periodontitis and cardiovascular disease has been reported in population studies.3

Based on Linda’s high hs-CRP, the practitioner tested Linda’s oral bacteria with the MyPerioPath® test from OralDNA® Labs. She wanted to see if Linda’s elevated biomarker of heart disease might have its origin in the mouth. MyPerioPath® methodology uses real-time polymerase chain reaction (RT-PCR or qPCR) to identify and quantify microbial DNA in an oral rinse specimen.

Figure 1. Linda’s first OralDNA®Labs MyPerioPath® report from June 2015, showing numerous oral pathogens, albeit at low levels.

Linda’s MyPerioPath® test showed the presence of eight different periodontal pathogens. Her oral microbiome showed low levels of four high-risk periodontal disease pathogens, three intermediate-risk periodontal disease pathogens, and low amounts of one low-risk periodontal disease pathogen. The oral pathogens detected were: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum/periodonticum, Prevotella intermedia, Campylobacter rectus, and Capnocytophaga species.

Linda was immediately started on a protocol to improve her oral health. Linda was already flossing daily and brushing her teeth twice daily. In addition, the practitioner instructed Linda to use a stainless-steel tongue scraper before brushing her teeth. She added GUM® soft picks, once per day, to her flossing regimen. Linda was also directed to add two drops of frankincense essential oil to her Waterpik™ and irrigate her gums once daily.

When Linda came back for her annual exam the following year, her hs-CRP had dropped by half. However, it was still elevated. Since her hs-CRP still hinted that there was unchecked inflammation, the clinician advised Linda to continue her protocol with the tongue scraper, the GUM® soft picks, and the frankincense essential oil in the Waterpik. But this time she told Linda to start taking ProbioMax® DDS (Xymogen, Orlando, FL) once per day, at bedtime. ProbioMax® DDS is a chewable probiotic that contains the beneficial oral bacterium Streptococcus salivarius DSM 14685.

Table 1. Levels of hs-CRP in the patient, “Linda,” over the course of treatment. hs-CRP levels dropped into the moderate risk range. A hs-CRP level < 1, 1-3, and >3 is low risk, moderate risk, and high risk, respectively, for global cardiovascular disease.1

Date hs-CRP (mg/L) Reference Ranges (mg/L)
  Low Risk Moderate Risk High Risk
6/3/2015 6.3 <1.0 1-3 >6.0
6/13/2016 3.5 <1.0 1-3 >6.0
7/5/2017 2.8 <1.0 1-3 >6.0


When Linda’s physician ordered her routine diagnostic testing the following year (after approximately two years of treatment), everything looked much better. Her hs-CRP was much improved and within the desired range (1-3), yet still not optimal (<1.0 mg/L). Likewise, her oral microbial profile showed improvement, with pathogenic bacteria decreased by half. A. actinomycetemcomitans, P. gingivalis, T. forsythia, and C. rectus were no longer detected at levels deemed to be clinically significant to represent the quantity on the bar graph. Where there were eight different bacteria present in her first test, her follow-up test showed only four. T. denticola and F. nucleatum were still positive. P. intermedia and Capnocytophaga species were still present but were reduced, compared to the first test. Linda’s hs-CRP was much better after treating her oral health and this corresponded with an improvement in her oral microbiome. This suggests that Linda’s systemic inflammation and risk of heart disease was reduced by lowering oral pathogen dysbiosis.

Figure 2. Patient’s follow-up OralDNA®Labs MyPerioPath® report from July 2017, showing dramatic reduction in oral pathogens after two years of treatment.


In this case, a 62-year-old woman without any other major health issues showed high cardiovascular disease risk due to an elevated hs-CRP. Even though she didn’t have any signs of gum disease, oral microbial DNA testing by RT-PCR showed low levels of eight oral pathogens. Integrative medical treatment over two years decreased serum hs-CRP from 6.3 to 2.8 mg/L and decreased the number of oral pathogens to four. By addressing oral dysbiosis and systemic inflammation, as measured by decreasing hs-CRP, this patient may have avoided full blown heart disease. Based on this case and the current evidence, it is recommended to measure and normalize oral bacterial pathogens in patients with elevated hs-CRP as a comprehensive approach to oral-systemic disease. With tongue scraping, soft pics, water flossing with Frankincense essential oil, and S. salivarious chewable probiotics, the clinician treated the oral microbiome, reduced inflammation, and thereby reduced the patient’s risk for stroke and heart attack.


Physician-Dentist Collaboration Form for patients with elevated hs-CRP, created by Mark Burhenne, DDS.


  1. Kamath DY, Xavier D, Sigamani A, Pais P. High sensitivity C-reactive protein (hsCRP) & cardiovascular disease: An Indian perspective. Indian J Med Res. 2015;142(3):261-268.
  2. Bansal T, Pandey A, D D, Asthana AK. C-Reactive Protein (CRP) and its Association with Periodontal Disease: A Brief Review. Journal of clinical and diagnostic research : JCDR. 2014;8(7):ZE21-24.
  3. Mysak J, Podzimek S, Vasakova J, Mazanek J, Vinsu A, Duskova J. C-reactive protein in patients with aggressive periodontitis. J Dent Sci. 2017;12(4):368-374.

**To learn more about becoming an OralDNA Provider: Text “OralDNA” to 31996**

Cass Nelson-Dooley MS, Ellie Campbell DO

Cass Nelson-Dooley MS, Ellie Campbell DO

Cass Nelson-Dooley MS: Cass Nelson-Dooley, MS, studied medicinal plants in the rain forests of Panama, in 2003 as a Fulbright Scholar, and then launched a career in science and natural medicine. She researched the pharmacology of medicinal plants at the University of Georgia and AptoTec, Inc, and then joined the innovators at Metametrix Clinical Laboratory and Genova Diagnostics. She enjoys teaching, presenting, writing, and researching how to address the underlying causes of disease, not just the symptoms. She has over a decade of experience teaching doctors about integrative and functional laboratory results. In 2013, she started Health First Consulting, LLC, a medical communications company with the mission to improve human health using the written word. Ms. Nelson-Dooley is the author of Heal Your Oral Microbiome and has published case studies, book chapters, and journal articles about natural medicine, nutrition, and laboratory testing.

Ellie Campbell DO: Dr. Campbell is a native Chicagoan with a BS/MS in biology from the University of Illinois and a DO degree from the Kirksville College of Osteopathic Medicine. She completed her Family Medicine residency at the Medical College of Georgia in Augusta and is board-certified by the American Academy of Family Medicine, by The American Board of Integrative Medicine, and in Integrative and Holistic Medicine.

Dr. Campbell currently runs a solo, concierge-style integrative and holistic family practice in Cumming GA. Office services include IV hydration and vitamins both by injection and IV, advanced diagnostic testing including assessment of adrenal and sex hormones, urine organic acids, mold plate testing, comprehensive digestive stool analysis, food sensitivity testing, advanced lipid, lipoprotein and vascular inflammation testing, She offers MyPerioPath testing for inflammatory oral bacteria, carotid ultrasound and CIMT, and breast thermography. She sees patients for primary care, prevention and wellness exams as well as management and reversal of chronic disease.

Dr. Campbell has specialty interests in interdisciplinary collaboration, root cause resolution of chronic health conditions, innovative medical practice design, functional and restorative medicine, Functional Neurology, prevention of stroke and heart attacks, Bio-Identical Hormone Replacement Therapy and Vitamin D. She frequently lectures at continuing medical education conferences. She mentors medical students and resident physicians at her office.

Since 2010, Dr. Campbell hosts monthly networking via The Consortium of Integrative Care Practitioners of Atlanta and frequently lectures to both lay groups and physician medical education conferences. She is a medical advisor to the social wellness network, and teaches doctors how to remedy practice overwhelm and reverse engineer the practice of their dreams by practicing functional medicine from within a concierge membership model via In her spare time, Dr Campbell enjoys live theater, gardening, hiking, travel, cooking and playtime with her husband and three college age daughters.
Cass Nelson-Dooley MS, Ellie Campbell DO

2 thoughts on “Systemic Inflammation, Elevated hs-CRP, and Oral Bacterial Pathogens Decrease After Two Years of Natural Dental Health Treatments: A Case Study

  1. Lia Tarantino says:

    The study did not mention if in fact the patient saw a DDS or hygienist for fm probings, X-rays or other diagnostics to check for perio disease. Only patient reported no signs of. Most people report no sign of LOL.

  2. The patient had routine dental cleanings with a holistic dentist. No symptoms or concerns were reported. This leads us to believe that the elevated hs-CRP and oral pathogens preceded the development of gingivitis or periodontitis.

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